Anti-Inflammatory Effect and Mechanism of the Green Fruit Extract of Solanum integrifolium Poir.
Figure 5
Contributions of MAPKs on the anti-inflammatory action of the methanol fraction of Solanum integrifolium (ME). (a) RAW264.7 macrophages were cultured with vehicle (0.1% DMSO) or LPS (10 ng/mL) in the presence of the indicated concentrations of methanol fraction (ME) for 1 h. Total cell lysates were prepared and the phospho-ERK, JNK, and P38 MAPK were detected by Western blotting using antibodies specific for phosphoprotein. The same blots were then stripped and reprobed with antibodies specific for pan protein. These blots are representative ones from one of three independent experiments. (b) RAW264.7 cells were pretreated for 30 min with inhibitor (U0126 and SP600125, 10 μM) followed by ME (0.1 mg/mL) and then LPS (10 ng/mL) challenge for 20 h, and the amount of NO produced in the medium was determined by the Griess reaction. Data were obtained from four independent experiments and are expressed as the mean ± SD. indicating significant differences from the respective LPS-treated group; ; indicate significant differences from respective inhibitor-untreated group.
