Identification of Virulence Factors Genes in Escherichia coli Isolates from Women with Urinary Tract Infection in Mexico

Table 2

PCR conditions for each gene.

Genes	Inicial denaturation; (°C/min)	Denaturation; (°C/s)	Annealing; (°C/s)	Extension; (°C/s)	Final extention (°C/min)	Cycles
ecpA	96/5	94/30	62/45	72/45	72/5	35
ecpR-B	95/5	95/30	57.5/33	72/90	75/5	35
fimH	96/5	94/30	65.5/30	72/30	72/5	35
papC	95/5	94/30	58.2/30	72/40	72/5	40
sfaS	95/5	94/30	64/30	72/25	72/5	35
fogG	95/5	95/30	64/40	72/30	72/5	30
sfa/focDE	95/5	94/30	65/30	68/40	72/3	35
cnf-1	95/5	94/30	65.5/30	72/30	72/5	35
hlyA	95/5	94/30	63/30	68/60	72/5	30
𝑀𝑢𝑙𝑡𝑖1^∗	95/5	94/30	67.1/30	68/160	72/5	30
𝑀𝑢𝑙𝑡𝑖2^∗∗	95/5	94/30	61.5/30	68/180	72/5	35
Rfc	95/5	95/30	62.5/30	72/60	75/5	40

*Multiplex 1 for genes fyuA, iutA e ibeA.**Multiplex 2 for genes cdtB, cvaC y traT.