The Effects of Omega-3 Fatty Acid Supplementation on Dexamethasone-Induced Muscle Atrophy
Figure 6
Protein expression in GA muscle samples from rats treated with n-3 PUFA or vehicle for 40 days and dexamethasone or vehicle (last 10 days). Relative optical density of total Akt (tAkt) and phosphorylated Akt (P-Akt) ((a) and (c)), total GSK3β (tGSK3β) and phosphorylated GSK3β (P-GSK3β) ((b) and (d)), and tAkt/P-Akt and tGSK3β/P-GSK3β ratios ((e) and (f), resp.). Bands representing tAkt, P-Akt, tGSK3, and P-GSK3β are illustrated in (g). α-Tubulin (55 kDa) was used as control. The numbers represent the mean ± S.E.M. CT = control group (), DX = dexamethasone group (), n-3 = n-3 PUFA group (), and DX + n-3 = dexamethasone + n-3 PUFA group (). Values represent means ± S.E.M., analyzed by Student’s t-test, #.
