Frequency and Clinical Implication of the R450H Mutation in the Thyrotropin Receptor Gene in the Japanese Population Detected by Smart Amplification Process 2
Figure 1
The primer annealing site design and amplification protocol for detecting the R450H mutation in TSHR by the SmartAmp2 method are shown. (a) Sequences and annealing sites for the SmartAmp2 primer set to detect the R450H mutation in TSHR are shown. An arrow indicates the nucleotide position of the R450H mutation in TSHR. TP: turn-back primer; FP: folding primer; BP: boost primer; and OP: outer primer. (b) Amplification curves generated with the SmartAmp2 primer set to detect the R450H mutation in TSHR using plasmid templates encoding pSVL-TSHR (wt) and pSVL-TSHR (R450H) are shown. (c) Direct sequencing results of the plasmid DNA templates pSVL-TSHR (wt) and pSVL-TSHR (R450H) are given.
