Liquiritigenin Induces Tumor Cell Death through Mitogen-Activated Protein Kinase- (MPAKs-) Mediated Pathway in Hepatocellular Carcinoma Cells

Figure 2

LQ induced cell damage in both HepG2 and PLC/RPL/5 cells. LQ dose-dependently reduced cell viability after 24 h treatment in hepatocellular carcinoma cells (a). 24 h LQ exposure resulted in an enhancement on LDH release (b) and caspase 3 activity (c). The expression of cleaved PARP was increased by presentation to LQ (200 μM and 400 μM) for 24 h and quantification data was normalized by GAPDH (d). LQ dose-dependently enhanced the apoptotic rate after 12 h incubation in the two cell lines (e). Data are expressed as percent of corresponding control cells and the means ± S.D. (𝑛=3). *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001 versus control cells.