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Reference number | Cell type | Medium/vessel | Culture condition | Cell number | Duration | Passage | CM processing |
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[7] | Hu-ESC-EC HUVEC | EBM2 | NA | NA | 5 days | NA | NA |
[13] | (i) Hu-ESC-CD133/KDR-EPC (ii) Hu-UCB-EPC | EGM-2 (Lonza)—15 mL 150 mm culture dish | NA | 80% | 48 hours | P5–8 | Conc. 50x 10 kD |
[16] [29] | Hu-ESC-MSC Hu-ESC-MSC | DMEM—insulin, transferrin, selenoprotein, FGF2, PDGF-AB, glutamine, and -ME | NA NA | NA NA | 3 days 3 days | ≥80 PD 80 PD | Conc. 25x 10 kD—220 nm 0.5 mg/mL protein |
[18] | Hu-ESC-MSC | Chemically defined medium | NA | NA | 3 days | 80 PD | Conc. 25x 10 kDa |
[9] | Hu-AF SC—Ckit (+) | MEM Six-well plate | 5% CO2 | 150.000 70% | 16 hours | NA | from 500.000 cells—1 mL—Conc. 80 µL |
[20] | 1-Hu-AF-MSC 2-hu-AF-MSC-HPL | DMEM 0.5% FBS 25 cm2 TC flask | 5% CO2 | 1.5 × 106 80% | 24 hours | P5–13 | Conc. 25x 3 kD |
[8] | (i) Hu-PB-MNC-EPC (ii) HUVEC | EBM2 (Lonza) 1% FBS | 1.5% O2, 5% CO | NA | 72 hours | NA | NA |
[15] | Porcine PB-MNC-EPC | Ex vivo 15 (Lonza)—VEGF 1 ng/mL—FC plate | 5% CO2 | From 30–40 mL PB | 48 hours | P0 | Centr. 600 g 5 min, 0.2 m—ice |
[12] | Hu-UC-MSC | M199 | 5% CO2 | NA | 24 hours | P3 | Conc.- 0.2 m |
[10] | (i) Hu-UCB-MNC-SC (endothelial + MSC) (ii) HUVEC | EGM-2–15 mL 150 mm culture dish | 5% CO2 | 80% | 48 hours | P5–8 | Conc. 50x—10 kD |
[11] | Hu-UCB-CD34-EPC | M199 basal medium | 5% CO2 | 1 × 106 | 24 hours | NA | Conc. |
[30] | (i) Hu-UCB-USSC (ii) mBM-MSC | Ultra CULTURE medium 7.5% BABLc serum | 5% CO2 | 60% | 48 hours | NA | NA |
[14] | Hu-BM-MSC | αMEM 10% FBS | 5% CO2 | 2 × 107/flask 60–70% | Till 60–70% | P5 | Conc. 50x—5 kD 5 flask 100 L |
[22] | Hu-BM-MSC | NA-0.05% BSA | NA | 2 × 106 | 24 hours | P3–7 | Conc. 25x—3 kD |
[26] | Hu-BM-MSC | DMEM-0.05% BSA | normoxia | 2 × 106 | 24 hours | P3–7 | Conc. 25x—3 kD |
[27] | Hu-BM-MSC | DMEM-0.05% BSA | 5% CO2 0.5% O2 | 2 × 106 → split 1 : 2 → confl | 24 hours | P3–7 | Conc. 25x 3 kD |
[17] | Hu-BM-MNC-stro-3-MPC | αMEM | NA | 1 × 106 MPC | NA | P5 | Conc. |
[25] | (i) Hu-BM-MSC (ii) Hu-BM-CD133 (iii) Hu-BM-p75 (iv) Hu-fibro | αMEM | 5% CO2 (i) 1% O2 (ii) 21% O2 | 1 × 106 90% | 48 hours | P4-5 | 0.2 m—80°C conc. 40x—5 kD |
[28] | Rat-BM-MSC | DMEM T75 flask | NA | 90% | 48 hours | P2–4 | Conc. 40x—10 kD: 10mL 250 µL 0.22 µm—80°C |
[19] | Rat BM-MSC | DMEM-0.05% BSA 10 mL | NA | 80–90% | 12 hours | P3-4 | Conc. 25x 3 kD |
[23] | (i) Hu-SC-EDT (ii) BM-MSC (Lonza) | DMEM | 5% CO2 | 4 × 105 | 48 hours | P3–5 | 3000 rpm—3 min supernatant |
[3] | Hu-AD-MSC | NA | Hypoxia | NA | NA | NA | Conc.-freeze dried |
[24] | Hu-AD-MSC | αMEM 70 mL | Spheroid 1% O2—5% CO2 | 4.2 × 107 | 2 days | NA | Centr. |
[6] | Hu-AD-SC | CRM/αMEM CRM—hu allo 10% αMEM—FBS 10% | 1% O2—5% CO2 | 2.5–3 × 105/mL—24 mL—150 cm dish | 2 days | Up to P5 | Centr. |
CRM/αMEM CRM—hu allo 10% αMEM FBS 10% | Spheroid 1% O2—5% CO2 | 6–12 × 105/mL—30 L → 70 mL flask |
[1] | Hu-AD-SC | NA | NA Reference [13, 14] | 1 × 105 →80% | 24 hours | NA | Centr. 300 g 5 min—220 nm |
[5] | Hu-AD-SC | DMEM/F12 | 2% O2 5% CO2 | 4 × 105 | 72 hours | P4-5 | Centr. 300 g 5’ 0.22 µm, 3 kD |
[23] | Hu-MSC | DMEM-0.05% BSA 15 mL—175 cm2 flask | NA | 1 × 106 70–80% | 24 hours | NA | Conc. 25x 3 kD |
[31] | Hu-MSC (cell line) | DMEM-10% FBS 5 mL | 5% CO2 | 70% | 48 hours | NA | 100,000 g—1 hour supernatant 0.22 µm |
[33] | Hu-MSC (Lonza) | DMEM | 5% CO2 | 70% | 48 hours | P3–9 | 4°C or −80°C |
[32] | Mouse-iPSC | NA | NA | NA | NA | NA | NA |
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