Review Article

Prospect of Stem Cell Conditioned Medium in Regenerative Medicine

Table 2

Cell type, medium, culture condition, cell number, duration, passage, and processing of conditioned medium.

Reference numberCell typeMedium/vessel Culture condition Cell numberDurationPassage CM processing

[7]Hu-ESC-EC
HUVEC
EBM2NANA5 daysNANA
[13](i) Hu-ESC-CD133/KDR-EPC
(ii) Hu-UCB-EPC
EGM-2 (Lonza)—15 mL
150 mm culture dish
NA80% 48 hoursP5–8Conc. 50x
10 kD
[16]
[29]
Hu-ESC-MSC
Hu-ESC-MSC
DMEM—insulin, transferrin, selenoprotein, FGF2, PDGF-AB, glutamine, and -MENA
NA
NA
NA
3 days
3 days
≥80 PD
80 PD
Conc. 25x 10 kD—220 nm 0.5 mg/mL protein
[18]Hu-ESC-MSCChemically defined mediumNANA3 days80 PDConc. 25x
10 kDa
[9]Hu-AF SC—Ckit (+)MEM
Six-well plate
5% CO2150.000
70%
16 hoursNAfrom 500.000 cells—1 mL—Conc. 80 µL
[20]1-Hu-AF-MSC
2-hu-AF-MSC-HPL
DMEM 0.5% FBS
25 cm2 TC flask
5% CO21.5 × 106
80%
24 hours P5–13Conc. 25x
3 kD
[8](i) Hu-PB-MNC-EPC
(ii) HUVEC
EBM2 (Lonza) 1% FBS1.5% O2,
5% CO
NA72 hoursNANA
[15]Porcine PB-MNC-EPCEx vivo 15 (Lonza)—VEGF 1 ng/mL—FC plate5% CO2From 30–40 mL PB 48 hoursP0 Centr. 600 g
5 min, 0.2 m—ice
[12]Hu-UC-MSCM1995% CO2NA24 hoursP3Conc.-
0.2 m
[10](i) Hu-UCB-MNC-SC
(endothelial + MSC)
(ii) HUVEC
EGM-2–15 mL
150 mm culture dish
5% CO280% 48 hoursP5–8 Conc. 50x—10 kD
[11]Hu-UCB-CD34-EPCM199 basal medium5% CO21 × 10624 hoursNAConc.
[30](i) Hu-UCB-USSC
(ii) mBM-MSC
Ultra CULTURE medium 7.5% BABLc serum5% CO260%48 hoursNANA
[14]Hu-BM-MSCαMEM 10% FBS5% CO22 × 107/flask 60–70%Till 60–70% P5Conc. 50x—5 kD
5 flask 100 L
[22]Hu-BM-MSCNA-0.05% BSANA2 × 10624 hours P3–7Conc. 25x—3 kD
[26]Hu-BM-MSCDMEM-0.05% BSAnormoxia2 × 10624 hoursP3–7Conc. 25x—3 kD
[27]Hu-BM-MSCDMEM-0.05% BSA5% CO2
0.5% O2
2 × 106
split 1 : 2 
→ confl
24 hoursP3–7Conc. 25x
3 kD
[17]Hu-BM-MNC-stro-3-MPCαMEMNA1 × 106 MPCNAP5Conc.
[25](i) Hu-BM-MSC
(ii) Hu-BM-CD133
(iii) Hu-BM-p75
(iv) Hu-fibro
αMEM5% CO2
(i) 1% O2
(ii) 21% O2
1 × 106
90%
48 hoursP4-50.2 m—80°C 
conc. 40x—5 kD
[28]Rat-BM-MSCDMEM
T75 flask
NA90%48 hoursP2–4Conc. 40x—10 kD: 10mL 250 µL
0.22 µm—80°C
[19]Rat BM-MSCDMEM-0.05% BSA
10 mL
NA80–90% 12 hoursP3-4Conc. 25x
3 kD
[23](i) Hu-SC-EDT
(ii) BM-MSC (Lonza)
DMEM5% CO24 × 10548 hoursP3–53000 rpm—3 min supernatant
[3]Hu-AD-MSCNAHypoxia NANANAConc.-freeze dried
[24]Hu-AD-MSCαMEM 70 mLSpheroid 1% O2—5% CO24.2 × 107 2 daysNACentr.
[6]Hu-AD-SCCRM/αMEM
CRM—hu allo 10%
αMEM—FBS 10%
1% O2—5% CO22.5–3 × 105/mL—24 mL—150 cm dish2 daysUp to P5Centr.
CRM/αMEM
CRM—hu allo 10%
αMEM FBS 10%
Spheroid
1% O2—5% CO2
6–12 × 105/mL—30 L → 70 mL flask
[1]Hu-AD-SCNANA
Reference [13, 14]
1 × 105 →80% 24 hoursNACentr. 300 g
5 min—220 nm
[5]Hu-AD-SCDMEM/F122% O2
5% CO2
4 × 10572 hoursP4-5Centr. 300 g 5’
0.22 µm, 3 kD
[23]Hu-MSCDMEM-0.05% BSA 15 mL—175 cm2 flaskNA1 × 106
70–80%
24 hoursNAConc. 25x 3 kD
[31]Hu-MSC (cell line)DMEM-10% FBS
5 mL
5% CO270%48 hoursNA100,000 g—1 hour supernatant
0.22 µm
[33]Hu-MSC (Lonza)DMEM5% CO270% 48 hoursP3–94°C or −80°C
[32]Mouse-iPSCNANANANANANA

Hu: human, ESC: embryonic stem cell, EC: endothelial cell, HUVEC: human umbilical vein endothelial cell, EPC: endothelial progenitor cell, AF: amniotic fluid, SC: stem cell, MSC: mesenchymal SC, HPL: hepatic progenitor-like cell, AD: adipose tissue derived, PB: peripheral blood, MNC: mononuclear cell, UC: umbilical cord, UCB: UC blood, m: mouse, BM: bone marrow, MPC: mesenchymal progenitor cell, fibro: fibroblast, EDT: exfoliated deciduous tooth, iPSC: induced pluripotent SC, TC: tissue culture, NA: not available, FBS: fetal bovine serum, allo: allogenic serum, BSA: bovine serum albumin. *Filtered 220 nm 10 nm 100 nm yielded 10–220 nm versus 10–100 nm (<1000 kD) versus 100–220 nm (>1000 kD). FC: fibronectin coated, P: passage, PD: population doubling, CM: conditioned medium, conc.: concentrated, centr.: centrifugation.