Novel Bifunctional Single-Chain Variable Antibody Fragments to Enhance Virolysis by Complement: Generation and Proof-of-Concept
Figure 5
Benchtop fermentation: expression analysis and binding capability. Expression of 48scFvSCR1920 in X33 cells was tested using high-density fermentation (a) under different conditions and subsequently tested for binding to MuLV-env on Mus dunni cells using flow cytometry (b). For FACS-analysis 48scFvSCR1920 derived from F4 was used. 10 µl from culture SN was loaded directly for SDS-PAGE analysis. F: fermentation run. Controls for FACS-analysis, see Supporting Information S1.
