Novel Bifunctional Single-Chain Variable Antibody Fragments to Enhance Virolysis by Complement: Generation and Proof-of-Concept
Figure 8
In silico analysis of binding 48scFv to envelope-protein of F-MuLV. (a) LC-Fv (purple) and HC-Fv (yellow) of 48scFv are connected by a (G4S)3-linker (green). (b) F-MuLV-gp70 (grey, data from pdb: 1AOL) and the main binding pocket (red) to its cellular receptor. (c) Docking calculation of 48scFv and gp70. Two SCR1920-models (light blue) were superimposed to the C-terminus of 48scFv illustrating the flexibility of the GS-linker (green) and the probable covering area (arrows). 3D-modelling of 48scFv (a) and SCR1920 ((c), light blue) was performed using I-TASSER [15]. Docking calculation of 48scFv and F-MuLV-gp70 was performed by ZDOCK [16].
