Novel Bifunctional Single-Chain Variable Antibody Fragments to Enhance Virolysis by Complement: Generation and Proof-of-Concept

Table 1

Summary of fermentation runs producing 48scFvSCR1920.

Fermentation (F); number	X33 expressing	pH during induction	Temperature; during induction	WCW^1 at induction	WCW end of run	yield^2 [mg/L]
F1	48scFvSCR1920	5	30°C	250	294	18
F2	48scFvSCR1920	5	22°C	177	238	65
F3	48scFvSCR1920	3	30°C	182	440	56
F4	48scFvSCR1920	3	22°C	208	297	130

1WCW: wet cell weight; ²yield after first purification round using expanded bed adsorption/Streamline-chelating matrix, yield concentration corresponds to mg per L culture volume.