Molecular Cloning and Characterization of Novel Phytocystatin Gene from Turmeric, Curcuma longa

Figure 3

(a) cDNA of CypC with 870 bp long was amplified using primers covering from 5′ end to 3′ end designed from the information obtained in 5′/3′ RACE-method (lane M: 100 bp DNA ladder, lane 1: CypCl). (b) Nucleotide and deduced amino acid sequences of the identified full-length cDNA of CypCl. A signal peptide (from amino acid position 1–29th) was predicted by using SignalP3.0 program. The cleavage site is between amino acids 29 and 30 (as indicated with black arrow). Several motifs of phytocystatin were detected in the full-length sequence, such as (i) one G residues towards N-terminal, (ii) LARFAVEEHN, (iii) QVVAG, (iv) PW dipeptide, and (v) SNSL. The respective motifs had been boxed.

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