Association of CHMP4B and Autophagy with Micronuclei: Implications for Cataract Formation

Figure 1

CHMP4B localizes to various types of intercellular bridges in interconnected cells. (a) Confocal micrographs of HeLa cells stained with CHMP4B, a-tubulin, and Hoechst. CHMP4B localizes to the intercellular bridge that links the cells (arrow). Scale bars: 10 μm. (b)–(d) Confocal micrographs of Hep2, MCF-7 and U2Os cells, stained with CHMP4B and Hoechst. CHMP4B localizes to the bridge between the cells in all the above cell lines (arrows). Scale bars: 10 μm. (e) Confocal micrographs of HeLa cells stained with CHMP4B, Aurora B and Hoechst. CHMP4B localizes to the intercellular bridge during cytokinesis (arrows). Scale bars: 10 μm. (f)-(g) Confocal micrographs of HeLa cells stained with CHMP4B, Aurora B and Hoechst. CHMP4B localizes to thin (scale bars: 5 μm) (f) and thick (scale bars: 10 μm) (g) bridges (arrows) independently of Aurora B localization.