Alkylation of Histidine Residues of Bothrops jararacussu Venom Proteins and Isolated Phospholipases : A Biotechnological Tool to Improve the Production of Antibodies
Figure 4
Inhibition of the myotoxic activity by antibodies against native and BPB-modified crude venom and myotoxins. (a) Myotoxic effect of crude venom or isolated PLA2 alone or following incubation with antibodies (50 : 1 antibody : toxin, w/w) in mice. The values were estimated by the levels of creatine kinase (CK) activity in plasma 3 h after the injection of samples. Phosphate-buffered saline (PBS) was included as a control. Histopathological analysis induced by myotoxins. Light micrographs of sections of mouse gastrocnemius muscle 24 h after injection of 50 μg of myotoxins (BthTX-I or BthTX-II) alone (b) or incubated with antibodies (50 : 1 antibody : toxin, w/w), dissolved in 50 μL PBS, and stained with hematoxylin and eosin. (c) Control mice were injected with PBS alone; (d) normal integer fibers are seen. Note the presence of myonecrosis (N) and an abundant inflammatory infiltrate (I).
