Next-Generation Technologies for Multiomics Approaches Including Interactome Sequencing

Figure 2

The type of primer used contains a barcoded region (indicated in grey, green, blue, yellow, and red), with four selection-round-specific bases. The reads generated using high-throughput sequencing are sorted according to their barcoded parts and mapped to known genomic sequences. Read frequencies of each genomic position are calculated for each selection round and used for determining the enriched regions. Using barcoded primers can reduce the risk of cross-contamination between libraries. Moreover, in a PPI analysis, increasing the sequencing depth can help detect contamination between samples. In the experiment shown, the Roche 454 Sequencer was used. Statistical significance was calculated by comparing the read frequencies with the frequencies of the initial library and the negative control.