Vitamin A Impairs the Reprogramming of Tregs into IL-17-Producing Cells during Intestinal Inflammation

Figure 2

Regulatory T cells generated under different polarizing conditions differentially express Treg markers but present similar suppressive capacity. FACS-sorted naive T cells from the OTII/Foxp3-GFP reporter mice were cultured with splenic APC and anti-CD3 in the presence of TGF-β plus IL-2 (TIL-2 Treg), plus RA (TRA Treg), or plus both RA and IL-2 (TILRA Treg). (a) Surface phenotype and Foxp3-GFP expression was analyzed by flow cytometry after 6 days of culture. Representative histograms of CD39, CD73, CTLA4, and LAG3 expression on Foxp3⁺ cells (upper graph). Average MFI ± SEM was derived from three independent experiments (lower graph). , , and . (b) Foxp3-GFP⁺ iTreg cells were sorted and cocultured with CellTrace Violet-labeled naive T cells at different effector , Treg ratios, and then stimulated with anti-CD3 plus CD11c⁺ DCs. Representative histograms of T effector cells proliferation (left). The graph represents the percentage of proliferation of CellTrace Violet-labeled Teff cells (right). Data are representative of three independent experiments.

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