Vitamin A Impairs the Reprogramming of Tregs into IL-17-Producing Cells during Intestinal Inflammation

Figure 3

IL-2 confers iTreg resistance to Th17 conversion, while RA and IL-2 cooperate to sustain Foxp3 expression following stimulation under Th17-polarizing conditions. FACS-sorted antigen-specific iTreg cells generated in the presence of TGF-β plus IL-2 (TIL-2 Treg), plus RA (TRA Treg), or plus both RA and IL-2 (TILRA Treg) were recultured under Th17-polarizing conditions with IL-6, TGF-β1, IL-1β, and α-IFNγ. Six days later, the cells were collected for intracellular IL-17 and Foxp3-GFP staining. (a) Representative staining showing the percentage of Foxp3-GFP expression versus IL-17 content. (b) Values indicate means ± SEM of four separate experiments. Repeated measure ANOVA with Bonferroni’s post test , , and .

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