Dragging Human Mesenchymal Stem Cells with the Aid of Supramolecular Assemblies of Single-Walled Carbon Nanotubes, Molecular Magnets, and Peptides in a Magnetic Field

Table 1

Lineage-specific differentiation induction media.

Medium	Media	Serum	Supplementation
Control	DMEM, 5 mM sodium bicarbonate (Cinética Química Ltda), penicillin (100 units/mL, Sigma-Aldrich), streptomycin (0.1 mg/mL, Sigma-Aldrich), amphotericin B (0.25 μg/mL, Sigma-Aldrich), gentamicin (60 mg/L, Schering-Plough).	10% FBS (Cripion Biotecnologia LTDA)	None
Adipogenic (AM)	DMEM, 5 mM sodium bicarbonate, penicillin (100 units/mL), streptomycin (0.1 mg/mL), amphotericin B (0.25 μg/mL), gentamicin (60 mg/L).	10% FBS	0.5 mM isobutylmethylxanthine (Sigma-Aldrich), 200 μM indomethacin (Sigma-Aldrich), 1 μM dexamethasone (Aché), and 10 μM insulin (Eli Lilly and Company).
Osteogenic (OM)	DMEM, 5 mM sodium bicarbonate, penicillin (100 units/mL), streptomycin (0.1 mg/mL), amphotericin B (0.25 μg/mL), gentamicin (60 mg/L).	10% FBS	50 μg/mL ascorbate-2-phosphate (Ecibra), 10 mM β-glycerophosphate (Sigma-Aldrich), and 0.1 μM dexamethasone (Aché).
Chondrogenic (CM)	DMEM, 5 mM sodium bicarbonate, penicillin (100 units/mL), streptomycin (0.1 mg/mL), amphotericin B (0.25 μg/mL), gentamicin (60 mg/L).	1% FBS	1 mM dexamethasone, 125 μg/mL de BSA (PAA), 1 mM pyruvate (Sigma-Aldrich), 200 U/mL insulin, 3.25 μg/mL transferrin (Wako), 0.01 μg/mL TGF-β1 (Sigma-Aldrich), and 5 mg/mL ascorbate-2-phosphate.