| Medium | Media | Serum | Supplementation |
| Control | DMEM, 5 mM sodium bicarbonate (Cinética Química Ltda), penicillin (100 units/mL, Sigma-Aldrich), streptomycin (0.1 mg/mL, Sigma-Aldrich), amphotericin B (0.25 μg/mL, Sigma-Aldrich), gentamicin (60 mg/L, Schering-Plough). | 10% FBS (Cripion Biotecnologia LTDA) | None |
| Adipogenic (AM) | DMEM, 5 mM sodium bicarbonate, penicillin (100 units/mL), streptomycin (0.1 mg/mL), amphotericin B (0.25 μg/mL), gentamicin (60 mg/L). | 10% FBS | 0.5 mM isobutylmethylxanthine (Sigma-Aldrich), 200 μM indomethacin (Sigma-Aldrich), 1 μM dexamethasone (Aché), and 10 μM insulin (Eli Lilly and Company). |
| Osteogenic (OM) | DMEM, 5 mM sodium bicarbonate, penicillin (100 units/mL), streptomycin (0.1 mg/mL), amphotericin B (0.25 μg/mL), gentamicin (60 mg/L). | 10% FBS | 50 μg/mL ascorbate-2-phosphate (Ecibra), 10 mM β-glycerophosphate (Sigma-Aldrich), and 0.1 μM dexamethasone (Aché). |
| Chondrogenic (CM) | DMEM, 5 mM sodium bicarbonate, penicillin (100 units/mL), streptomycin (0.1 mg/mL), amphotericin B (0.25 μg/mL), gentamicin (60 mg/L). | 1% FBS | 1 mM dexamethasone, 125 μg/mL de BSA (PAA), 1 mM pyruvate (Sigma-Aldrich), 200 U/mL insulin, 3.25 μg/mL transferrin (Wako), 0.01 μg/mL TGF-β1 (Sigma-Aldrich), and 5 mg/mL ascorbate-2-phosphate. |
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