Molecular Characterization of a New Alkaline-Tolerant Xylanase from Humicola insolens Y1

Table 1

Effect of 5 mM metal ions and chemical reagents on the xylanase activity of Xyn11B.

Chemicals	Relative activity (%)^a	Chemicals	Relative activity (%)
None	100.0 ± 1.2	Ca^2+	95.7 ± 1.2
Mn^2+	110.4 ± 2.8	Cr^3+	93.0 ± 0.6
Na^+	101.6 ± 1.3	Pb^2+	91.6 ± 1.8
Li^+	100.6 ± 1.4	Cu^2+	87.0 ± 2.3
Co^2+	100.6 ± 0.9	Ag^+	76.5 ± 2.1
Zn^2+	99.6 ± 2.2	Hg^2+	0
K^+	99.2 ± 0.4	β-Mercaptoethanol	160.6 ± 4.7
Fe^3+	98.7 ± 1.6	EDTA	96.1 ± 1.1
Mg^2+	96.6 ± 0.8	SDS	28.2 ± 3.2

Values represent the mean ± SD ( = 3) relative to untreated samples.