CK2 Secreted by Leishmania braziliensis Mediates Macrophage Association Invasion: A Comparative Study between Virulent and Avirulent Promastigotes

Table 1

Effect of casein on the secreted CK2 activities of Leishmania braziliensis avirulent and virulent samples.

Systems	Virulent strain	Avirulent strain	Virulent activity/avirulent; activity
	pmol·Pi mg^−1·min^−1; ± E.P.	pmol·Pi mg^−1·min^−1; ± E.P.
Constitutive ecto-CK2 activity (eCK2)	34.400 ± 1.340	0.040 ± 0.001	860.00
Constitutive intracellular CK2 activity (iCK2)	4.800 ± 0.610	0.290 ± 0.002	16.55
Constitutive secreted CK2 activity (CsCK2)	7.290 ± 0.320	0.060 ± 0.006	121.50
Inducible secreted CK2 activity (IsCK2)	13.960 ± 1.010^*	0.070 ± 0.006	199.43

The reactions were performed as described in Section 2. The values represent the mean of 3 independent experiments conducted in triplicate (* indicates P ≤ 0.005 in relation to CsCK2; one-way ANOVA, Tukey’s post hoc test).