CK2 Secreted by Leishmania braziliensis Mediates Macrophage Association Invasion: A Comparative Study between Virulent and Avirulent Promastigotes

Table 3

Effect of polyamines on the constitutive and inducible secreted CK2 activity of the Leishmania braziliensis virulent strain.

Addition	Constitutive secreted CK2		Inducible secreted CK2
	nmol Pi/mg·min^−1; ±SE	% kinase activity	nmol Pi/mg·min^−1; ±SE	% kinase activity
None	7.70 ± 0.63	100.00	13.96 ± 0.64	100.00
Putrescine (500 μM)	14.48 ± 0.52^**	188.01	21.50 ± 1.15^**	154.01
Spermidine (500 mM)	18.00 ± 0.84^**	233.77	25.32 ± 0.24^*	181.38
Spermine (500 mM)	26.75 ± 0.72^*	347.40	32.76 ± 0.26^*	234.67

The reactions were performed as described in Section 2. The values are presented as the mean of 3 independent experiments conducted in triplicate (* indicates P ≤ 0.0002, ** indicates P ≤ 0.007, and *** indicates P ≤ 0.05 compared with control without addition; one-way ANOVA, Tukey’s post hoc test).