Serum Caveolin-1 as a Novel Biomarker in Idiopathic Pulmonary Artery Hypertension

Figure 1

Specificity of capture and detection antibodies to rCav-1 and native Cav1 of human serum. Recombinant Cav1 protein was purified using affinity chromatography and analyzed by SDS-PAGE (I) and immunoblotting (II and III). In panel I, the rCav-1 protein migrated as a single band and displayed >95% purity by Coomassie blue staining. The binding specificity of the capture and detection antibodies to rCav-1 and human serum was demonstrated and showed in panel II and panel III, respectively. Numbers on the left indicate sizes of protein markers (lane M).