Statistical Optimization of Laccase Production and Delignification of Sugarcane Bagasse by Pleurotus ostreatus in Solid-State Fermentation

Table 4

Effect of inorganic and organic nitrogen sources and different inducers, Cu²⁺ and ferulic acid (Fer), on the level of laccase activity produced by the strain P.  ostreatus 22 Em after 5 days of fermentation on sugarcane bagasse.

N source (g/L)	Inducer	U/g dry substrate
Ammonium sulfate
2.5	CuSO4 150 M	9.942 ± 1.97
Yeast extract
2.5	0	2.970 ± 0.651
2.5	CuSO4 150 M	44.23 ± 2.44
2.5	CuSO4 150 M + Fer 2 mM	89.18 ± 3.95
7.5	CuSO4 150 M	56.25 ± 5.08