Statistical Optimization of Laccase Production and Delignification of Sugarcane Bagasse by Pleurotus ostreatus in Solid-State Fermentation

Table 5

Results of laccase activity obtained for the central composite design experiments after 5 days of solid-state fermentation on sugarcane bagasse.

	Variables			Response
	Yeast extract g/L	CuSO4  M	Ferulic acid mM	Activity U/g
1	4	75	1	61.30
2	10	75	1	51.02
3	4	225	1	98.12
4	10	225	1	58.80
5	4	75	3	50.90
6	10	75	3	46.60
7	4	225	3	68.80
8	10	225	3	65.60
9	1.96	150	2	82.19
10	12.04	150	2	52.75
11	7	24	2	62.33
12	7	276	2	35.91
13	7	150	0.32	48.03
14	7	150	3.68	89.43
15	7	150	2	158.8
16	7	150	2	149.4

Note: enzyme activities in units per gram of dry substrate.