Statistical Optimization of Laccase Production and Delignification of Sugarcane Bagasse by Pleurotus ostreatus in Solid-State Fermentation

Table 7

Experiments for the verification of the predicted model of laccase production after 5 days of solid-state fermentation on sugarcane bagasse.

Yeast extract (g/L)	CuSO4 (M)	Ferulic acid (mM)	Predicted (U/g)	Experimental (U/g)
4.0	75	1.0	64.04	79.74 ± 7.94
5.5	112.5	1.5	133.2	114.7 ± 7.17
6.4	172.6	1.86	161.3	155.3 ± 5.92
7.0	150	2.0	156.6	149.2 ± 4.95
8.5	187.5	2.5	134.2	122.9 ± 5.12
10	225	3.0	66.01	95.17 ± 7.75

Note: enzyme activities in units per gram of dry substrate.