Evaluation of APP695 Transgenic Mice Bone Marrow Mesenchymal Stem Cells Neural Differentiation for Transplantation

Figure 3

Autophagy flux was impaired in APP695 MSCs. APP695 and WT MSCs-n (neuronally differentiated counterparts of APP695 MSCs) were cultured in complete medium plus 10 μmol/L rapamycin for the indicated times and then subjected to immunocytochemical staining (a) and western blot (b, d) using anti-LC3 antibody. (c, e) Cells were cultured as in (a) and p62 expression levels were analyzed by Western blot. LC3 II/LC3 I ratio increased in both APP695 and WT cells (^* versus zero hr) but more pronounced in APP695 (^# versus WT).

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