Review Article
Research Progress on the Role of ABC Transporters in the Drug Resistance Mechanism of Intractable Epilepsy
Table 2
Immunohistochemical localization of P-gp in rat brains: comparison of different fixation and staining protocols.
| | Protocol |
Pretreatment (ethanol/acetate) | Antibody | Control rats | Rats after kainate induced SE | | Astrocytes | Endothelial cells | Neurons | Astrocytes | Endothelial cells | Neurons |
| | #1 (acetone fixed cryostat sections of snap-frozen tissue) | − | C219 | − | + | − | − | + | (+) | | − | mdr-(Ab-1) | − | + | − | − | + | + | | − | H241 | − | (+) | − | − | + | (+) |
| | #2 (paraformaldehyde-fixed cryosections) | − | C219 | − | (+) | − | − | (+) | − | | − | mdr-(Ab-1) | (+) | (+) | − | + | (+) | − | | − | H241 | − | (+) | − | − | (+) | − | | + | C219 | − | + | − | − | + | − | | + | mdr-(Ab-1) | − | + | − | (+) | + | − | | + | H241 | − | + | − | − | + | − |
| | #3 (paraformaldehyde-fixed vibratome sections) | − | C219 | − | − | − | + | − | − | | − | mdr-(Ab-1) | (+) | (+) | − | + | (+) | − | | − | H241 | − | − | − | (+) | − | − | | + | C219 | − | + | − | − | + | − | | + | mdr-(Ab-1) | − | + | − | (+) | + | − | | + | H241 | − | + | − | − | + | − |
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Consistent immunostaining of P-gp in a specific cell type is indicated by +, weak staining by (+), and a lack of staining by −.
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