|
Species | Agent and method | Promoter | Reporter gene | Life stage transfected | Transfection type | References |
|
S. mansoni | RNA and plasmid by particle bombardment | Spliced Leader | Luciferase | Adult worm | Transient | [34] |
Plasmid by particle bombardment | Hsp70 | GFP | Adult worm and sporocysts | Transient | [35] |
Plasmid by particle bombardment | ER60 | GFP | Female miracidia with sporocysts | Transient | [36] |
Plasmid by particle bombardment | SmCNA | GFP | Adult worm | Transient | [37] |
Plasmid by particle bombardment | ER60 | GFP | Adult worm | Transient | [38] |
Plasmid by particle bombardment | Hsp70 | EGFP | Miracidia | Transient | [39] |
RNA by electroporation | — | Luciferase | Schistosomula | Transient | [40] |
RNA by particle bombardment and electroporation | — | Luciferase | Sporocysts, miracidia, and adult worm | Transient | [41] |
VSVG-pseudo MMLV plasmid by cation polybrene | SL and hsp70 | EGFP and Luciferase | Schistosomula | Transient | [42] |
Electroporation | SmACT1.1 | Luciferase | Schistosomula | Transient | [43] |
PiggyBac by electroporation | Actin and HSP70 | Luciferase | Schistosomula | Stable | [44] |
VSVG-pseudo MMLV plasmid by lipofectamine | Sma-Zinc | Luciferase | Adult worm and schistosomula | Stable | [45] |
RNA and VSVG-pseudo MMLV by electroporation | — | CY3 and luciferase | Eggs | Stable | [46] |
MLV pseudotyped plasmid by lipofectamine or polyethylenimine | MLV 5′, Pol II, vasa-like, Actin, Pol III U6 | Luciferase and EGFP | Schistosomula, eggs, and adult worms | Stable | [47] |
|
S. japonicum | Plasmid by electroporation | CMV | EGFP and luciferase | Schistosomula and adult worm | Transient | [48] |
VSVG-pseudo pantropic retrovirus plasmid by cation polybrene | LTR | hTERT | Schistosomula | Stable | [49] |
|