A Novel Insight into Dehydroleucodine Mediated Attenuation of Pseudomonas aeruginosa Virulence Mechanism

Figure 4

Effect of DhL on P. aeruginosa biofilm formation. Overnight cultures of PAO1 were resuspended in fresh AB medium (control) and in AB medium supplemented with DhL (see Section 2) in PVC tubes. After 24 hr of incubation at 30°C, the biofilms on the wall of the tubes were visualized by staining with a crystal violet solution. Biofilm formation was quantified by measuring OD₅₄₆ of crystal violet-stained wells rinsed with ethanol. Each column is the mean of three individual experiments with two replicates per treatment. Significant (asterisk indicated ) decrease in biofilm formation was observed in PAO1 when treated with DhL. Data represent the mean of three independent experiments ± SEM.