Research Article
A Validated TLC-Densitometric Method for the Determination of Mesterolone in Bulk Material and in Tablets
Table 1
Chromatographic characteristic of mesterolone analyzed using different NP-TLC plates and chloroform-acetone (40 : 10, v/v) as mobile phase.
| Mobile phase | Chromatographic plates | | Visualization mode | Densitometric analysis without the use of visualizing agent | Densitometric analysis after derivatizing with 10% sulphuric acid | Densitometric analysis after derivatizing with 10% phosphomolybdic acid (PMA) | [nm] | [nm] | [nm] |
| Chloroform-acetone 40 : 10 (v/v) | Silica gel 60 Art. 1.05553 (E. Merck) | 0.66 ± 0.01 | 200 | 467 | 745 | Silica gel 60F254 Art. 1.05554 (E. Merck) | 0.75 ± 0.02 | 200 | 467 | 745 | Silica gel 60/Kieselguhr F254 Art. 1.05567 (E. Merck) | 0.87 ± 0.01 | 248 | 468 | 745 |
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