A Heparan Sulfate-Binding Cell Penetrating Peptide for Tumor Targeting and Migration Inhibition

Figure 3

Inhibitory effect of CPPecp on HUVEC migration. HUVECs were seeded onto the upper side of transwell insert membrane containing CPPecp at indicated concentration at 37°C for 4 h. The lower side of transwell was filled with complete EC medium supplementing with 20 ng/mL VEGF. Migrated cells on the lower surface of transwell insert membrane were stained with Hoechst (a). Percentage of migrated cells in the presence of VEGF and set as 100% (positive control). Alternation of HUVEC migration activity in the presence of VEGF and various concentrations of CPPecp were quantified as compared with positive control (b). The data represents means ± SD (standard deviation) of three independent experiments. ^*; ^**; ^*** compared with control. Magnification: 100x. Scale bar: 400 μm.

(a)

(b)