Research Article

Selection of the Strain Lactobacillus acidophilus ATCC 43121 and Its Application to Brewers’ Spent Grain Conversion into Lactic Acid

Table 3

Lactic acid production from agricultural residue hydrolysates.

Waste typeWaste pretreatmentEnzymatic hydrolysisFermentation conditionsYield of glucose after enzymatic hydrolysis Microorganism used in fermentation stepProduction levels of lactic acidYield of lactic acid per glucose consumedReferences

Brewer’s spent grain1.25% (v/v) sulfuric acid solution in a 1 : 8 g : g solid : liquid ratio, at 120°C for 17 minutes;
2% (v/v) sodium hydroxide solution in a 1 : 20 g : g solid : liquid ratio, at 120°C for 90 minutes
2.24% (v/v) cellulase (Novozymes) and 1% (v/v) β-glucosidase (Novozymes) using 8% (w/v) substrate, at 45°C and 120 rpm for 72 hoursInoculum with 10% (v/v) of cell suspension, at 37°C and 60 ×g75.0 g/LLactobacillus acidophilus ATCC 431218.9 g/L from AAT BSG 1
12.26 g/L from AAT BSG 1 + yeast extract
17.49 g/L from AAS BSG 2
22.16 g/L from AAS BSG 2 + yeast extract
0.48 g/g from AAT BSG 1
0.6 g/g from AAT BSG 1 + yeast extract
0.52 g/g from AAS BSG 2
0.61 g/g from AAS BSG 2 + yeast extract
This study

Mango peelCoupling of the microbial hydrolysis and fermentation of the carbohydrate substrate into a single step at 35°C under static incubation conditionsConsortium of indigenous microorganisms of mango peels17.48 g/L[17]

Municipal solid waste (MSW)2% (v/v) of H2SO4 (1 : 10 solid-to-liquid ratio) at 124°C for 15 minutesOptimum conditions: initial pH 7.6, 1% v/v inoculum, and 5% (w/v) calcium carbonate buffer at 32°C41.3 g/LLactobacillus arabinosus B-787, 
L. arabinosus B-788, 
L. arabinosus B-813, 
L. arabinosus B-531, 
L. pentosus B-277, 
L. pentosus B-473, 
L. plantarum USDA 422, and L. xylosus B-4449
~17–19 g/L excepted for L. xylosus B-4449 ~6 g/LFrom 0.52 g/g to 0.73 g/g[18]

Brewer’s spent grain1.25% (v/v) sulfuric acid solution in a 1 : 8 g : g solid : liquid ratio, at 120°C for 17 minutes;
2% (v/v) sodium hydroxide solution in a 1 : 20 g : g solid : liquid ratio, at 120°C for 90 minutes
Celluclast 1.5 L from Trichoderma reesei (Novozymes, Denmark)Inoculated with an initial cell concentration of 1.0 g/L; statical incubation at 37°C50.0 g/LLactobacillus delbrueckii7.87 g/L using nonsupplemented BSG hydrolysate
~9 g/L using BSG hydrolysate supplemented with yeast extract
0.7 g/g[19]

Corn cobs5, 10, and 15% (v/v) of H2SO4 autoclaved at different temperatures for different intervals of timeOptimum conditions: 40°C, pH 5-6 4.0 (% w/w)Lactobacillus delbrueckii25.62 g/L[20]