Purification and Characterization of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus penicillioides Isolate and Its Potential for Souse with Detergents

Table 2

Effect of various metal ions and additives on the activity of the enriched -amylase from A. penicillioides TISTR 3639.

Additives (2 mM)	Relative activity (%)
None	100^∗f
BaCl2	97.8 ± 0.8^e
CaCl2	104.2 ± 1.7^g
FeCl2	78.1 ± 1.5^c
HgCl2	97.1 ± 1.4^e
MgCl2	99.1 ± 2.2^ef
ZnCl2	44.3 ± 0.9^a
-Mercaptoethanol	95.1 ± 1.5^d
EDTA	73.4 ± 0.4^b

One hundred percent activity corresponded to the activity of the amylase without additive. Data are shown as the mean relative value ± 1SD, derived from three repeats. Means followed by a different lowercase superscript letter (a, b, c, d, e, f, and g) are significantly different (ANOVA and DMRT of the transformed data, ).