Improving the Thermostability and Optimal Temperature of a Lipase from the Hyperthermophilic Archaeon Pyrococcus furiosus by Covalent Immobilization

Table 2

Activity of different immobilized preparations of P.  furiosus lipase in the hydrolysis of different substrates in pH 7.0.

Support	Activity (U/g) in different substrates
	1	2	3	4
	10 mM	5 mM	5 mM	0.5 mM
Octyl-agarose	2982	0.12 (2.39)	3.64	0 (0.03)
Glyoxyl-DTT-agarose	2259	3.08 (0.84)	17.83	0.03 (0.29)
Glyoxyl-agarose	5188	1.01 (0.29)	4.81	0.05 (0.22)

Activity was measured at pH 5.