Improving the Thermostability and Optimal Temperature of a Lipase from the Hyperthermophilic Archaeon Pyrococcus furiosus by Covalent Immobilization

Table 3

Enantioselectivity of different immobilized preparations of P.  furiosus lipase in the hydrolysis of 2 at 25°C pH = 7.0.

Supports	ee (%)		Preference
Octyl-agarose	35 (8.6)	2 (1.2)	S (S)
Glyoxyl-DTT-agarose	91 (46)	22 (2.7)	R (R)
Glyoxyl-agarose	53 (46)	3.2 (2.7)	R (R)

Activity was measured at pH 5.