Improving the Thermostability and Optimal Temperature of a Lipase from the Hyperthermophilic Archaeon Pyrococcus furiosus by Covalent Immobilization

Table 4

Enantioselectivity of different immobilized preparations of P.  furiosus lipase in the hydrolysis of 4 at 25°C pH = 7.0.

Supports	ee (%)		Preference
	pH 7	pH 7	pH 7
Octyl-agarose	65 (21)	4.6 (1.5)	R (R)
Glyoxyl-DTT-agarose	7.4 (61)	1.16 (4.2)	S (R)
Glyoxyl-agarose	27 (52)	1.7 (3.1)	R (R)

Activity was measured at pH 5.