SERCA2 Haploinsufficiency in a Mouse Model of Darier Disease Causes a Selective Predisposition to Heart Failure

Figure 7

Effect of Atp2a2 heterozygosity on markers of ER stress and apoptosis in HCM and HCM/HET hearts. Heart RNA from WT mice, mice expressing the Glu180Gly mutant α-tropomyosin (HCM), and HCM/Atp2a2^+/− double mutant (HCM/HET) mice was analyzed by RT-PCR. Panels (a)–(e) compare WT and HCM; panels (f)–(j) compare HCM and HCM/HET. mRNA levels are shown for (a, f) ER chaperones BiP/GRP78 (Hspa4) and GRP94 (Hsp90b1); (b, g) PERK (Eif2ak3); (c, h) CHOP (Ddit3); (d, i) caspase 12 (Casp12); and (e, j) HCLS1 associated protein X-1 (Hax1) in WT, HCM, and HCM/HET hearts. mRNA levels were normalized to Gapdh. Values shown are means ± SE. = at least 4 for each genotype. versus WT controls; versus HCM.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)