Roles of ER and GPR30 in Proliferative Response of Human Bladder Cancer Cell to Estrogen

Figure 1

Proliferation of T24 cell was promoted by E2. (a) qPCR analysis of expression of estrogen receptor in T24 cells. ERα mRNA was rarely expressed in the cells, and the relative expression levels of ERβ and GPR30 were 0.21 and 0.35, respectively (normalization to β-actin). (b) Expression of estrogen receptors in human T24 bladder cancer cells. Twenty micrograms of whole protein extracts was used for western blot analysis. ERα was not detected. (c) Cell proliferation promoted by E2. T24 cells were seeded in 96-well plates at a density of approximately 2 × 10³ for each well and incubated with E2; then the OD values were examined after 0, 24, 48, 72, and 96 h by MTT assays. 0.1% DMSO was used as the negative control. The values represent the mean ± SD of the data from three independent experiments. ; .

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