Oxidative Stress Alters miRNA and Gene Expression Profiles in Villous First Trimester Trophoblasts

Table 4

Altered miRNAs with putative altered mRNAs targets after 4 h exposure to H₂O₂ in villous 3A trophoblasts.

miRNA name	Number of genes targeted by this miRNA	Number of target sites identified in target genes	Range of strength scores^a	Target genes
hsa-miR-181a-5p	3	3	−0.4593 to −0.234	DCN, IL1A, TNF
hsa-miR-181c-5p	3	3	−0.4573 to −0.219	DCN, IL1A, TNF
hsa-miR-181b-5p	3	3	−0.4593 to −0.234	DCN, IL1A, TNF
hsa-miR-181d-5p	3	3	−0.4573 to −0.234	DCN, IL1A, TNF
hsa-miR-27b-3p	3	3	−0.203 to −0.0872	IGF1, LEP, LPL
hsa-miR-29a-3p	3	4	−0.4102 to −0.094	IGF1, LEP, LPL
hsa-miR-29c-3p	3	4	−0.4102 to −0.09	IGF1, LEP, LPL
hsa-miR-875-3p	3	4	−0.504 to −0.2443	CD40LG, NDRG1, TNF
hsa-miR-9-5p	3	3	−0.453 to −0.0467	IGF1, LEP, NDRG1
hsa-miR-454-3p	2	3	−0.3152 to −0.219	IGF1, TNF
hsa-miR-543	2	2	−0.39 to −0.259	ANGPT2, IL1A
hsa-miR-30e-5p	2	2	−0.452 to −0.1342	IGF1, IL1A
hsa-miR-30b-5p	2	2	−0.452 to −0.1236	IGF1, IL1A
hsa-miR-30a-5p	2	2	−0.452 to −0.1342	IGF1, IL1A
hsa-miR-30c-5p	2	2	−0.452 to −0.1236	IGF1, IL1A
hsa-miR-30d-5p	2	2	−0.452 to −0.1342	IGF1, IL1A
hsa-miR-424-5p	1	1	−0.0766 to −0.0766	IGF1
hsa-miR-152-3p	1	1	−0.3972 to −0.3972	IGF1
hsa-miR-148b-3p	1	1	−0.3972 to −0.3972	IGF1
hsa-miR-425-5p	1	1	−0.4961 to −0.4961	IGF1
hsa-miR-16-5p	1	1	−0.0977 to −0.0977	IGF1
hsa-miR-497-5p	1	1	−0.0766 to −0.0766	IGF1
hsa-miR-490-5p	1	1	−0.197 to −0.197	NTRK2
hsa-miR-193a-5p	1	1	−0.115 to −0.115	NTRK2
hsa-miR-151a-5p	1	1	−0.401 to −0.401	NTRK2
hsa-miR-330-3p	1	2	−0.05 to −0.01	NTRK2
hsa-miR-17-5p	1	2	−0.149 to −0.148	NTRK2
hsa-miR-1271-5p	1	1	−0.108 to −0.108	NDRG1
hsa-miR-20a-5p	1	2	−0.17 to −0.148	NTRK2
hsa-miR-154-5p	1	1	−0.263 to −0.263	NTRK2
hsa-miR-26b-5p	1	1	−0.1205 to −0.1205	IGF1
hsa-miR-766-3p	1	1	−0.161 to −0.161	IGF1
hsa-miR-190a-5p	1	1	−0.1862 to −0.1862	IGF1
hsa-miR-452-5p	1	1	−0.3111 to −0.3111	IGF1
hsa-miR-320a	1	1	−0.1466 to −0.1466	IGF1
hsa-miR-320b	1	1	−0.1466 to −0.1466	IGF1
hsa-miR-18b-5p	1	1	−0.2185 to −0.2185	IGF1
hsa-miR-374a-5p	1	1	−0.511 to −0.511	CCL2
hsa-miR-26a-5p	1	1	−0.105 to −0.105	IGF1
hsa-miR-222-3p	1	1	−0.1612 to −0.1612	IGF1
hsa-miR-221-3p	1	1	−0.1612 to −0.1612	IGF1
hsa-miR-192-5p	1	1	−0.2211 to −0.2211	IGF1
hsa-miR-196a-5p	1	1	−0.0267 to −0.0267	IGF1
hsa-let-7f-5p	1	1	−0.1633 to −0.1633	IGF1
hsa-let-7a-5p	1	1	−0.1592 to −0.1592	IGF1
hsa-miR-196b-5p	1	1	−0.0267 to −0.0267	IGF1
hsa-let-7b-5p	1	1	−0.1592 to −0.1592	IGF1
hsa-let-7i-5p	1	1	−0.1592 to −0.1592	IGF1
hsa-let-7e-5p	1	1	−0.1592 to −0.1592	IGF1
hsa-let-7d-5p	1	1	−0.1612 to −0.1612	IGF1
hsa-let-7c-5p	1	1	−0.1592 to −0.1592	IGF1
hsa-let-7g-5p	1	1	−0.1592 to −0.1592	IGF1
hsa-miR-576-5p	1	1	−0.1892 to −0.1892	IGF1

 ^aStrength scores are the scores derived from the TargetScan algorithm. A more negative number indicates a stronger score and an increased likelihood that the gene is a bona fide target for the miRNA evaluated.