Evaluation of the Ribosomal Protein S1 Gene (rpsA) as a Novel Biomarker for Mycobacterium Species Identification
Table 3
Comparison of mycobacteria identified by 16S rDNA/ITS/hsp65/rpoB gene sequencing and by rpsA sequencing among the 188 clinical isolatesa.
Primary identificationb ()
Primary identificationb ()
First choice
Second choice
Identification ()
%match
Identification ()
%match
M. intracellulare (75)
M. intracellulare (4)
100%
M. avium (4)
95.6%
M. intracellulare (1)
99.8%
M. avium (1)
95.3%
M. intracellulare (53)
98.9%
M. avium (53)
95.1%
M. intracellulare (10)
98.7%
M. avium (10)
94.9%
M. intracellulare (1)
98.5%
M. avium (1)
95.3%
M. intracellulare (3)
98.1%
M. avium (3)
94.7%
M. intracellulare (1)
97.5%
M. avium (1)
93.2%
M. intracellulare (2)
97.1%
M. avium (2)
96.1%
M. abscessus (39)
M. abscessus (9)
100%
M. abscessus subspecies massiliense (9)
98.7%
M. abscessus (10)
99.8%
M. abscessus subspecies massiliense (10)
98.5%
M. abscessus subspecies massiliense (20)
99.4%
M. abscessus (20)
99.1%
M. kansasii (23)
M. kansasii (22)
100%
M. asiaticum (22)
96.3%
M. szulgai (1)
96.6%
M. marinum/M. ulcerans (1)
96.0%
M. avium (10)
M. avium subspecies paratuberculosis (5)
99.6%
M. avium (5)
99.4%
M. avium subspecies paratuberculosis (4)
99.8%
M. avium (4)
99.2%
M. avium subspecies paratuberculosis (1)
97.5%
M. avium (1)
97.0%
M. fortuitum complex (17)
M. fortuitum (2)
100%
M. senegalense/M. thermoresistibile (2)
98.1%
M. fortuitum (11)
99.8%
M. porcinum (11)
98.5%
M. fortuitum (1)
99.6%
M. porcinum (1)
98.3%
M. fortuitum/M. porcinum (1)
98.9%
M. senegalense/M. thermoresistibile (1)
98.3%
M. thermoresistibile/M. senegalense (2)
99.6%
M. fortuitum (2)
98.1%
M. gordonae (7)
M. gordonae (1)
99.2%
M. asiaticum (1)
95.5%
M. gordonae (2)
98.5%
M. asiaticum (2)
96.3%
M. gordonae (3)
95.9%
M. asiaticum (3)
95.5%
M. szulgai (1)
95.9%
M. gordonae (1)
94.7%
M. tuberculosis complex (16)
M. tuberculosis (2)
100%
M. canetti (2)
99.8%
M. tuberculosis (12)
99.8%
M. canetti (12)
99.6%
M. tuberculosis (2)
99.6%
M. canetti (2)
99.4%
M. neoaurum (1)
M. neoaurum (1)
98.7%
M. smegmatis (1)
95.4%
Numbers in parentheses () represent the numbers of isolates identified as a particular species. bIdentification based on sequencing of at least two of the following: 16S rDNA, 16-23S rRNA gene internal transcribed spacer (ITS), and rpoB and hsp65 genes.
