Case Report Associated with Aspergillosis and Hepatitis E Virus Coinfection in Himalayan Griffons

Figure 5

PCR assays of tissues with primers specific for fungi and HEV. (a) Fungi: lane M, DL2000 marker; 1, griffon 1 heart; 2, griffon 1 spleen; 3, griffon 1 kidney; 4, griffon 2 spleen; 5, griffon 2 lung; 6, griffon 2 kidney; 7, griffon 3 spleen; 8, griffon 3 liver; 9, griffon 3 lung; 10, griffon 3 heart; 11, griffon 4 liver; 12, griffon 4 kidney; 13, griffon 4 lung; 14, griffon 4 spleen; 15, griffon 4 heart; 16, negative control; 17, negative control; 18, lane M, DL2000 marker. The fungi amplicon was 425 bp. (b) HEV: positive for griffon 2 liver and griffon 1 kidney. The HEV amplicon was 348 bp.

(a)

(b)