Production by Tobacco Transplastomic Plants of Recombinant Fungal and Bacterial Cell-Wall Degrading Enzymes to Be Used for Cellulosic Biomass Saccharification

Figure 2

Southern blot hybridization to show site-specific integration of introduced transgenes into tobacco plastid genome for the representative transplastomic plant. The partial coding region of rbcL and accD was used to show the stable and site specific integration of transgenes. Gene specific DNA probe was also used to confirm the stable integration of the transgene. Note the lack of any untransformed plastid DNA in the transplastomic lines. Molecular marker (M), wild type (1), and transformed (2) plants.