Research Article

Production by Tobacco Transplastomic Plants of Recombinant Fungal and Bacterial Cell-Wall Degrading Enzymes to Be Used for Cellulosic Biomass Saccharification

Table 2

Activity of various tobacco chloroplast expressed enzymes on carboxymethylcellulose (CMC) and on microcrystalline cellulose (MCC) substrates.

EnzymeActivity U/mg proteinTypeGeneration of plants used in the study
CMCMCC

Cel630.3 ± 0.020.5 ± 0.03ExoglucanaseT3
Cel30.36 ± 0.020.12 ± 0.005EndoglucanaseT3
CelK13.6 ± 0.150.2 ± 0.03EndoglucanaseT3
Cel60.16 ± 0.030.4 ± 0.05ExoglucanaseT1
Cel70.25 ± 0.0090.21 ± 0.03EndoglucanaseT1