Decreased Splenic T-Lymphocytes in Apolipoprotein M Gene Deficient Mice

Figure 1

Generation of apoM deficient mice. (a) Replace apoM with a loxP-Neo-loxP cassette. (b) Southern blot analysis of 5′ and 3′ probes. (c) Results of Southern blot analyses of ES cells B5, H9, B10, and C11. B6 genomic DNA as negative control. (d) Genotyping generations of heterozygote/heterozygote and wild type crosses were performed by PCR of mouse tail DNA. Lanes 2, 9, 13, and 14 only showed bands at 558 bp, which was identified as apoM gene wild type; lanes 3, 4, 6, 11, and 12 only showed bands at 342 bp, which was identified as apoM gene deficient homozygotes; lanes 5, 7, 8, and 10 showed both 342 and 558 bp bands, which were identified as apoM gene heterozygotes.

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