Subtoxic Doses of Cadmium Modulate Inflammatory Properties of Murine RAW 264.7 Macrophages

Figure 2

Influence of Cd on the cell viability of RAW 264.7 macrophages. Cell viability was determined using the WST-1 assay (Roche Diagnostics). 2 × 10⁵ cells/well were seeded in a 96-well microculture plate and subsequently stimulated with various Cd concentrations alone or in the presence of hk S.E. After 24 and 48 h WST-1 Reagent was added for 1 h followed by determination of the optical density in supernatants at 437 nm using a conventional microplate reader (Saphire2, Tecan). Data represent the mean values and are representative of three independent experiments ( per experiment). indicates significant () differences compared to control.

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