BioMed Research International / 2015 / Article / Tab 1 / Research Article
Vitamin K1 Exerts Antiproliferative Effects and Induces Apoptosis in Three Differently Graded Human Colon Cancer Cell Lines Table 1 The polyamine profile: putrescine (Put), spermidine (Spd), spermine (Spm), and total polyamine (Total) in Caco-2, HT-29, and SW480 cell lines following administration of vitamin K1 (VK1) increasing concentrations (from 10
M to 200
M) after 24 h, 48 h, and 72 h.
Caco-2 Control VK1 10 M VK1 50 M VK1 100 M VK1 200 M 24 h Put 0.70 ± 0.06 0.34 ± 0.03 0.30 ± 0.05 Spd 12.17 ± 0.44 12.30 ± 0.95 9.43 ± 0.35 Spm 11.63 ± 0.33 12.07 ± 0.52 10.87 ± 0.29 11.53 ± 0.26 11.33 ± 0.67 Total 24.50 ± 0.72 24.71 ± 0.39 20.60 ± 0.62 48 h Put 0.87 ± 0.07 0.57 ± 0.07 Spd 16.47 ± 0.33 10.53 ± 0.37 9.63 ± 0.38 Spm 13.07 ± 0.69 12.73 ± 0.48 12.13 ± 0.47 12.60 ± 0.49 11.97 ± 0.022 Total 30.41 ± 0.83 23.57 ± 0.74 72 h Put 0.57 ± 0.04 0.48 ± 0.05 Spd 10.53 ± 0.32 9.72 ± 0.40 Spm 15.83 ± 0.35 14.93 ± 0.46 Total 26.94 ± 0.70 25.13 ± 0.92 HT-29 Control VK1 10 M VK1 50 M VK1 100 M VK1 200 M 24 h Put 0.76 ± 0.05 0.65 ± 0.04 0.53 ± 0.13 0.55 ± 0.10 0.58 ± 0.08 Spd 13.04 ± 0.05 12.67 ± 0.18 13.03 ± 0.12 12.93 ± 0.18 12.76 ± 0.23 Spm 9.70 ± 0.32 9.93 ± 0.28 9.60 ± 0.63 10.13 ± 0.54 9.97 ± 0.24 Total 23.49 ± 0.39 23.27 ± 0.35 23.30 ± 0.76 23.71 ± 0.55 23.38 ± 0.47 48 h Put 1.41 ± 0.11 1.14 ± 0.23 1.06 ± 0.18 Spd 10.11 ± 0.27 9.43 ± 0.24 9.45 ± 0.19 Spm 14.40 ± 0.47 14.47 ± 0.20 13.40 ± 0.40 Total 25.92 ± 0.81 25.04 ± 0.55 23.91 ± 0.43 72 h Put 0.53 ± 0.18 0.52 ± 0.08 0.30 ± 0.03 Spd 8.44 ± 0.29 8.46 ± 0.31 7.50 ± 0.43 Spm 13.50 ± 0.32 10.50 ± 0.46 8.30 ± 0.32 Total 22.47 ± 0.79 19.48 ± 0.84 16.10 ± 0.66 SW480 Control VK1 10 M VK1 50 M VK1 100 M VK1 200 M 24 h Put 2.24 ± 0.20 1.45 ± 0.09 1.03 ± 0.14 Spd 13.27 ± 0.26 11.74 ± 0.14 9.73 ± 0.32 Spm 12.93 ± 0.29 12.03 ± 0.20 10.77 ± 0.29 Total 28.44 ± 0.75 25.22 ± 0.43 21.53 ± 0.75 48 h Put 1.59 ± 0.17 1.04 ± 0.18 0.79 ± 0.11 Spd 9.93 ± 0.29 8.73 ± 0.39 7.60 ± 0.40 Spm 13.13 ± 0.40 13.05 ± 0.62 12.16 ± 0.20 Total 24.65 ± 0.52 22.82 ± 0.45 20.55 ± 0.72 72 h Put 1.79 ± 0.11 1.57 ± 0.14 1.65 ± 0.09 Spd 7.31 ± 0.21 6.58 ± 0.25 6.27 ± 0.17 Spm 12.58 ± 0.23 12.18 ± 0.18 12.38 ± 0.38 Total 21.68 ± 0.55 20.33 ± 0.46 20.30 ± 0.64
compared to control cells.