Expression of Heterologous Cellulases in Thermotoga sp. Strain RQ2

Figure 9

Stabilities of the E. coli recombinant strains. The bacterial cultures were transferred for four consecutive times with 100 μg mL⁻¹ ampicillin and were subject to plasmid extraction (a) and Congo red plate assays (showing DH5α/pHX02 as an example) (b). Samples used for plate assays were prepared from normalized cultures. +, C. saccharolyticus DSM 8903; LB, Luria-Bertani broth.

(a)

(b)