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Strain or plasmid | Description | Reference |
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E. coli | | |
DH5α | F− endA1 hsdR17 (rk−, mk+) supE44 thi-1 λ− recA1 gyrA96 relA1 deoR Δ(lacZYA-argF)-U169ϕ80dlacZΔM15 | [24] |
Thermotoga | | |
T. sp. strain RQ2 | Isolated from geothermally heated sea sediment, Ribeira Quente, Săo Miguel, Azores. | [25] |
T. maritima MSB8 | The first type strain of Thermotoga, isolated from geothermally heated sea sediments, Porto di Levante, Vulcano, Italy. | [25] |
Caldicellulosiruptor | | |
C. saccharolyticus DSM 8903 | Isolated from wood in the flow of geothermal spring, Taupo, New Zealand. | [14] |
Plasmids | | |
pDH10 | Thermotoga-E. coli shuttle vector; Apr, | [23] |
pDH26 | pDH10-derived, with BsaI site erased; Apr, Kanr | This study |
pDH27 | pDH26-derived, with NdeI site erased; Apr, Kanr | This study |
pHX01 | pDH27-derived, having lacZ residual sequence removed; Apr, Kanr | This study |
pHX02.1 | Promoter and signal peptide region of TM1840 (amyA) were inserted into the NotI-SacI sites of pHX01; Apr, Kanr | This study |
pHX02 | Coding region (without the signal peptide) of Csac_1078 (celB) was inserted into BsaI-SacI sites of pHX02.1; Apr, Kanr | This study |
pHX04.1 | Promoter and signal peptide region of TM0070 (xynB) were inserted into the NotI-SacI sites of pHX01; Apr, Kanr | This study |
pHX04 | Coding region (without the signal peptide) of Csac_1078 (celB) was inserted into the BsaI-SacI sites of pHX04.1; Apr, Kanr | This study |
pHX07.1 | Promoter and signal peptide region of TM0070 (xynB) were inserted into the XhoI-PstI sites of pHX01; Apr, Kanr | This study |
pHX07 | Coding region (without the signal peptide) of Csac_1076 (celA) was inserted into the BsaI-PstI sites of pHX07.1; Apr, Kanr | This study |
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