Research Article
Expression of Heterologous Cellulases in Thermotoga sp. Strain RQ2
Table 3
Nucleotide sequences of primers used in this study.
| Primer | Sequence and restriction sites |
| DBs F | 5′ATCATGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGC3′ BsaI | DBs R | 5′ATCGTCGGTCTCTACCGCGGGAACCACGCTCACCGGCTCCAGATTTATCAGC3′ BsaI | DNd F | 5′ATCGTCGGTCTCTATATGCATGTGCACCAAACCACTTTGAGTACGTTCCCG3′ BsaI | DNd R | 5′ATCGTCGGTCTCCATATATTATTTAGAGGACCTTATATTCCCCAAGATTGG3′ BsaI | DLZ F | 5′GTCTGACTAGTGCAACGCATGCGAGGTTCTAGAGATTAGGGTGATGGTTCACGTAGTGG3′ SphI | DLZ R | 5′GACACGCATGCCGACGGCCAGTGAATTGTAATACGAC3′ SphI | AmP F | 5′CGAGGAACGAAGCGGCCGCGGACACCTCCTTTAGATTACAAAGAGTTTAC3′ NotI | AmP R | 5′GACTTAGAGCTCGATGACGGTCTCACTGGGCTAGTACCATCTGTGTTTGTGCTGTTTG3′ SacI BsaI | XyBPB F | 5′CGAGGAACGAAGCGGCCGCGAAAACTCACCTCCCTTGATTGTATG3′ NotI | XyBPB R | 5′GACTTAGAGCTCGATGACGGTCTCACTGGAGAGCTGAAAACTGGAACACATCCCAAC3′ SacI BsaI | XyBPA F | 5′CGAGGACTCGAGGAAAACTCACCTCCCTTGATTGTATG3′ XhoI | XyBPA R | 5′GATTAGCTGCAGGATGACGGTCTCAATGGAGAGCTGAAAACTGGAACACATCCCAAC3′ PstI BsaI | CelB F | 5′GACGACGGTCTCACCAGACTGGAGTATTCCAAGTTTATG3′ BsaI | CelB R | 5′GGCGCGGAGCTCTCATCAGTGATGGTGATGGTGATGTTTTGAAGCTGGAACTGGCTCAGGCTCATTATTG3′ SacI | CelA F | 5′GACTACGAAGACATCCATGGCAGGAGGCTAGGGCTGGTTC3′ BbsI | CelA R | 5′GGCGCGCTGCAGTCATCAGTGATGGTGATGGTGATGTTGATTACCGAACAGAATTTCATATGTTG3′ PstI | CelBV F | 5′AGACGCGATGGGACATATCTATCCGGTATGG3′ | CelBV R | 5′GAAGCTGGAACTGGCTCAGGCTCATTATTG3′ |
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