Expression of Heterologous Cellulases in Thermotoga sp. Strain RQ2

Table 3

Nucleotide sequences of primers used in this study.

Primer	Sequence and restriction sites
DBs F	5′ATCATGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGC3′; BsaI
DBs R	5′ATCGTCGGTCTCTACCGCGGGAACCACGCTCACCGGCTCCAGATTTATCAGC3′; BsaI
DNd F	5′ATCGTCGGTCTCTATATGCATGTGCACCAAACCACTTTGAGTACGTTCCCG3′; BsaI
DNd R	5′ATCGTCGGTCTCCATATATTATTTAGAGGACCTTATATTCCCCAAGATTGG3′; BsaI
DLZ F	5′GTCTGACTAGTGCAACGCATGCGAGGTTCTAGAGATTAGGGTGATGGTTCACGTAGTGG3′; SphI
DLZ R	5′GACACGCATGCCGACGGCCAGTGAATTGTAATACGAC3^′; SphI
AmP F	5′CGAGGAACGAAGCGGCCGCGGACACCTCCTTTAGATTACAAAGAGTTTAC3′; NotI
AmP R	5′GACTTAGAGCTCGATGACGGTCTCACTGGGCTAGTACCATCTGTGTTTGTGCTGTTTG3′; SacI       BsaI
XyBPB F	5′CGAGGAACGAAGCGGCCGCGAAAACTCACCTCCCTTGATTGTATG3′; NotI
XyBPB R	5′GACTTAGAGCTCGATGACGGTCTCACTGGAGAGCTGAAAACTGGAACACATCCCAAC3′; SacI       BsaI
XyBPA F	5′CGAGGACTCGAGGAAAACTCACCTCCCTTGATTGTATG3′; XhoI
XyBPA R	5′GATTAGCTGCAGGATGACGGTCTCAATGGAGAGCTGAAAACTGGAACACATCCCAAC3′; PstI       BsaI
CelB F	5′GACGACGGTCTCACCAGACTGGAGTATTCCAAGTTTATG3′; BsaI
CelB R	5′GGCGCGGAGCTCTCATCAGTGATGGTGATGGTGATGTTTTGAAGCTGGAACTGGCTCAGGCTCATTATTG3^′; SacI
CelA F	5′GACTACGAAGACATCCATGGCAGGAGGCTAGGGCTGGTTC3^′; BbsI
CelA R	5′GGCGCGCTGCAGTCATCAGTGATGGTGATGGTGATGTTGATTACCGAACAGAATTTCATATGTTG3′; PstI
CelBV F	5′AGACGCGATGGGACATATCTATCCGGTATGG3′
CelBV R	5′GAAGCTGGAACTGGCTCAGGCTCATTATTG3′