A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21^Waf1/CIP1 Expression

Figure 1

dsP21-397 induces p21 expression by targeting a complementary promoter sequence in BC cells. (a) Schematic representation of the p21 promoter and dsP21-397 target site. Sequence of the miR-1180-5p target site located at nucleotide -397 relative to the transcription start site. (b and c) Expression of p21 and GAPDH mRNA levels was assessed by real-time PCR. GAPDH served as a loading control. T24 and EJ cells were transfected with 50 nM of the indicated dsRNAs for 72 h. mock sample was transfected in the absence of dsRNA. compared to mock and dsControl groups. (d) Induction of p21 protein expression was detected by Western blot analysis. GAPDH levels were also detected and served as a loading control.

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